DDEL-18. PEG-AU-BP NANOCARRIERS FOR DBTRG HUMAN GLIOMA CELLS

Abstract n-butylidenephthalide (BP) has been verified to have the superior ability of cancer cell toxicity. Furthermore, gold nanoparticles (Au) are biocompatible materials as well effective carrier deliver bio-active molecular for therapeutics. In present research, Au were firstly conjugated with polyethylene glycol (PEG), and cross-linked BP obtain PEG-Au-BP nanocarriers. The physicochemical properties characterized through Ultraviolet-visible spectroscopy (UV-Vis), Fourier-transform infrared (FTIR) Dynamic light scattering (DLS) confirm combination PEG,Au,and BP. Through iv vitro, normal BAEC cells DBTRG human glioma treated nanocarriers investigate cytotoxicity, uptake efficiency, mechanism endocytic routes. According results MTT assay, could significantly inhibit proliferation. Cell efficiency potential cellular transportation in both lines observed be higher at time point 2hr 24 hr. Moreover, mechanisms endocytosis, clathrin-mediated endocytosis autophagy, explored routes absorb Next, progression apoptosis after treatment was investigated by flow cytometry. figured out that remarkably regulate cycle Sub-G1 phase induced more apoptotic cells. expression apoptotic-related proteins determined. After PEG-Au-BP, cascade proteins, p21, BAX, Act-caspase-3 expressed vivo, tissue morphology particle distribution mice examined retro-orbital sinus injection demonstrated integrity brain (forebrain, midbrain, cerebellum), other organs did not show significant destruction. above finding elucidated a nanodrug tumor therapies.